Mouse Alkaline Sphingomyelinase ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Alkaline Sphingomyelinase in biofluid samples, such as tissue homogenates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . Alk present in samples reacts with an anti-Mouse Alk capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-Alk detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active Alk in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse Alk. Detection Target: Mouse Alkaline Sphingomyelinase Reactivity: Mouse Range: 0.156-10ng/mL Sensitivity: 0.051ng/mL Storage Conditions: Store all components at 4┬░C This Mouse Alk activity assay is for the quantitative determination of Alkaline Sphingomyelinase in samples including tissue homogenates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.